Pettenkofer Test
Objective This test is used to prove the presence of bile salt. Base Theory Bile salt will reacts with hydroxymethylenephurphural to forms red solution. Hydroxymethylenephurphural is formed of sugar that dehidrated by sulphuric acid. Procedure Dissolve few crystals of saccharose in 1 ml of diluted bile salt solution. Sublayer this solution with 1 ml of concentrated H 2 SO 4 (adding sulphuric acid pouring on the wall of the tube). Red ring is formed between both layers.