Ninhydrin Test

Objective
Ninhydrin reaction is chemical reaction to detect existence of amino acids.

 Base Theory
Ninhydrin (triketohydrindene hydrate) is an oxidating agent which leads to the oxidative deamination of alpha-amino groups. It is very important for the detection and the quantitative analysis of amino acids. Ninhydrin also reacts with primary amines however the formation of carbon dioxide is quite diagnostic for amino acids. When reacting with free amines from the amino acid, blue or purple color is produced. Most of the amino acids are hydrolyzed and reacted with ninhydrin except proline. When ninhydrin reacts with amino acids, the reaction also releases CO2. A ninhydrin solution is commonly used by forensic investigators in the analysis of latent fingerprints on porous surfaces such as paper. Amino acid containing fingermarks, formed by minute sweat secretions which gather on the finger’s unique ridges, are treated with the ninhydrin solution which turns the amino acid finger ridge patterns purple and therefore visible.

 Procedure
Get 5 drops  0,1% ninhidrin’s solution in test tube and add with 2 ml matter which will be tested. Warming in boiler approximately in 10 minutes.
Blue color indicate positive reaction of Ninhydrin Test

Komentar

Posting Komentar

Postingan populer dari blog ini

Pettenkofer Test

Objective This test is used to prove the presence of bile salt. Base Theory Bile salt will reacts with hydroxymethylenephurphural to forms red solution. Hydroxymethylenephurphural is formed of sugar that dehidrated by sulphuric acid. Procedure Dissolve few crystals of saccharose in 1 ml of diluted bile salt solution. Sublayer this solution with 1 ml of concentrated H 2 SO 4  (adding sulphuric acid pouring on the wall of the tube). Red ring is formed  between both layers.
Baca selengkapnya

Biuret Test

Gambar
Objective The biuret test is a chemical test used for detecting the presence of peptide bonds. Base Theory In the presence of peptides, a copper(II) ion forms a violet-colored complex in an alkaline solution.Several variants on the test have been developed. The Biuret reaction can be used to assay the concentration of proteins because peptide bonds occur with the same frequency per amino acid in the peptide. The intensity of the color, and hence the absorption at 540 nm, is directly proportional to the protein concentration, according to the Beer-Lambert law. In spite of its name, the reagent does not in fact contain biuret ((H2N-CO-)2NH). The test is so named because it also gives a positive reaction to the peptide bonds in the biuret molecule. Procedure An aqueous sample is treated with an equal volume of 1% strong base (sodium or potassium hydroxide most often) followed by a few drops of aqueous copper(II) sulfate. If the solution turns purple, protein is present. 5–160 m...
Baca selengkapnya

Ingenhousz Experiment

Gambar
Objective To prove the photosynthesis result is O 2 Base theory Plants as the autotroph organism have ability to produce organic matter from anorganic matter. This ability because plants have organel called chloroplast. In the daylight, the sunshine reach the chloroplast and plants can transform H2O and CO2 into carbohydrate and O2. Carbohydrate will be saved as food reserves and to build cell wall. The reaction of photosynthesis: 6H 2 O + 6CO 2 ----> C 6 H 12 O 6  + 6O 2 Tools and materials test tube bekker glass (1000 ml) funnel water plants  matches Procedure of experiment Fill the bekker glass with 800 ml of water. Prepare the plant, put the plant in the funnel. Enter the funnel and the plant into bekker glass with inverted. Enter the tip of the funnel into the test tube. Look at the picture! Place the bekker glass in the outside (there is sunshine). Waiting for 15 minutes. Lift the test tube and closed its hole with your hand. Li...
Baca selengkapnya