Pettenkofer Test

Objective
This test is used to prove the presence of bile salt.

 Base Theory
Bile salt will reacts with hydroxymethylenephurphural to forms red solution. Hydroxymethylenephurphural is formed of sugar that dehidrated by sulphuric acid.

 Procedure
Dissolve few crystals of saccharose in 1 ml of diluted bile salt solution. Sublayer this solution with
1 ml of concentrated H2SO4 (adding sulphuric acid pouring on the wall of the tube). Red ring is formed  between both layers.

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